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Your Position: Home > All Other Proteins > MICA > MIA-H5253

Human MICA Protein, Fc Tag (MALS verified)

  • Synonym
    MIC-A
  • Source
    Human MICA, Fc Tag (MALS verified) (MIA-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 24- Gln 308 (Accession # AAH16929.1).
    Predicted N-terminus: Glu 24
  • Molecular Characterization
    Online(Glu 24- Gln 308) AAH16929.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 59.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human MICA, Fc Tag (MALS verified) (Cat. No. MIA-H5253) SDS-PAGE gel

Human MICA, Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human MICA, Fc Tag (MALS verified)Human MICA, Fc Tag (MALS verified) (Cat. No. MIA-H5253) ELISA bioactivity

Immobilized Human NKG2D, His Tag (Cat. No. NKD-H5245) at 2 μg/mL (100 μL/well) can bind Human MICA, Fc Tag (Cat. No. MIA-H5253) with a linear range of 0.5-2 ng/mL (QC tested).

Human MICA, Fc Tag (MALS verified)Human MICA, Fc Tag (MALS verified) (Cat. No. MIA-H5253) ELISA bioactivity

Immobilized Human MICA, Fc Tag (Cat. No. MIA-H5253) at 1 μg/mL (100 μL/well) can bind Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) with a linear range of 0.6-20 ng/mL (Routinely tested).

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