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Your Position: Home > All Other Proteins > BCMA > BCA-C82E5

Biotinylated Cynomolgus / Rhesus macaque BCMA / TNFRSF17 Protein, His,Avitag™

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag (BCA-C82E5) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 53 (Accession # G7Q0I4-1).
    Predicted N-terminus: Met 1
    Request for sequence
  • Molecular Characterization
    Online(Met 1 - Ala 53) G7Q0I4-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 9.6 kDa. The protein migrates as 15-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag (Cat. No. BCA-C82E5) SDS-PAGE gel

Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Request for HPLC data
Bioactivity-ELISA
Biotinylated Cynomolgus / Rhesus macaque BCMA, His,AvitagBiotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag (Cat. No. BCA-C82E5) ELISA bioactivity

Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag (Cat. No. BCA-C82E5) with a linear range of 0.2-1 ng/mL (QC tested).

Protocol
Biotinylated Cynomolgus / Rhesus macaque BCMA, His,AvitagBiotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag (Cat. No. BCA-C82E5) ELISA bioactivity

Immobilized Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag (Cat. No. BCA-C82E5) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.2-5 ng/mL (Routinely tested).

Protocol
  • Background
    Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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