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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCM-R52W5

Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (SPR verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Rabbit FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (SPR verified) (FCM-R52W5) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Val 297 (FCGRT) & Val 22 - Tyr 120 (B2M) (Accession # ABY47597.1(FCGRT) & ALR85722.1(B2M)).
    Predicted N-terminus: Ala 24 (FCGRT) & Val 22 (B2M)
  • Molecular Characterization
    Online(Ala 24 - Val 297 (FCGRT) & Val 22 - Tyr 120 (B2M)) ABY47597.1 (FCGRT) & ALR85722.1 (B2M)

    Rabbit FCGRT&B2M Heterodimer Protein, His Tag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.1 kDa (FCGRT) and 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 34 kDa (FCGRT) and 12 kDa (B2M) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rabbit FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (SPR verified) (Cat. No. FCM-R52W5) SDS-PAGE gel

Rabbit FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Rabbit FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-R52W5) captured on CM5 Chip via anti-His antibody can bind Herceptin® with an affinity constant of 2.12 μM as determined in SPR assay (Biacore T200) (QC tested).

  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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