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Your Position: Home > Recombinant Antibodies > S protein RBD > S1N-M130

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130)

  • Source
    Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric MAb (S1N-M130) is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV-2 Spike S1 Protein.
  • Isotype
    Human IgG1/kappa
  • Specificity
    This product binds SARS-CoV-2 Spike RBD as well as SARS-CoV Spike RBD. The cross-reactivity with other coronaviruses has not been tested yet.
  • Application
    This antibody can be paired with other Anti-SARS-CoV-2 Spike S1 antibodies to detect SARS-CoV-2 Spike S1 protein in sandwich ELISA or LFA assay.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20 to -70°C for 12 months in lyophilized state from date of receipt;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric mAb (Cat. No. S1N-M130) SDS-PAGE gel

Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric mAb on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric mAbAnti-SARS-CoV-2 Spike S1 Antibody, Chimeric mAb (Cat. No. S1N-M130) ELISA bioactivity

Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H2) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike S1 Antibody, chimeric mAb (Cat. No. S1N-M130) with a linear range of 0.08-2.5 ng/mL (QC tested).

Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric mAbAnti-SARS-CoV-2 Spike S1 Antibody, Chimeric mAb (Cat. No. S1N-M130) ELISA bioactivity

Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike S1 Antibody, chimeric mAb (Cat. No. S1N-M122) at 2 μg/mL (100 μL/well) can bind S protein RBD. And then add Biotinylated Anti-SARS-CoV-2 Spike S1 Antibody (Cat. No. S1N-M130) at 1:5000. Detection was performed using HRP-conjugated streptavidin with sensitivity of 25 pg/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric mAb (Cat. No. S1N-M130) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 0.0576 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $380.00

Price(USD) : $2500.00

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