T The protein-based Blocking Buffer can be use in binding Assay of serum sample, to help reducing the background signal, but if you need capture sample to do MS, you need to do some follow-up.
a) Dilute your sample with the Blocking Buffer, add the diluted sample to Beads, incubate for 60 minutes at room temperature.
b) Wash the beads, and separated the beads from your samples afterwards using a magnetic plate.
c) If you need to detection the binding signal, dilute your detection antibody with the blocking buffer and add to the beads, if you want to dissociate the sample from the beads, add an suitable Regeneration Buffer to the sample bonded beads, and remain the dissociation sample supernatant.
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