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Your Position: Home > Viral Proteins > S protein > SPN-C52H2
  • Synonym
    S protein,Spike glycoprotein,S glycoprotein,COVID-19
  • Source
    SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (SPN-C52H2) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(R683A, R685A)) trimer Design.
    Predicted N-terminus: Val 16
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 138.0 kDa. The protein migrates as 180-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH5.5.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;

    2. -70°C for 3 months under sterile conditions.

  • Shipping
    This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (Cat. No. SPN-C52H2) SDS-PAGE gel

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (Cat. No. ) MALS images

The purity of SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H2) was more than 85% and around 550-680 kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified)SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (Cat. No. SPN-C52H2) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (Cat. No. SPN-C52H2) with a linear range of 0.9-31 ng/mL (QC tested).

  • Background
    It's been reported that Coronavirus  can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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