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Your Position: Home > Recombinant Antibodies > DNP > DNP-FM1

FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control

  • Source
    Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control was purified from HEK293 cell culture. It is the FITC labeled form of Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1).
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1-3.
  • Application
    This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-FM1) SDS-PAGE gel

FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of FITC-Labeled Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-FM1), and FITC signal was used to evaluate the binding activity (QC tested).

FACS assay shows that recombinant Biotinylated FACS

5e5 of PBMCs were stained with a series of concentrations of FITC-Labeled Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-FM1), and FITC signal was used to evaluate the binding activity (QC tested).

FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-FM1) and positive control respectively, and FITC signal was used to evaluate the binding activity (Routinely tested).

  • Background
    A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $290.00

Price(USD) : $1250.00

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