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Your Position: Home > Antibody titer assay kits > S protein RBD > TAS-K013

Anti-SARS-CoV-2 Antibody IgM Serologic Assay kit (Spike RBD protein)

For research use only.
Materials Provided
TAS013-C01Anti-human lgM antibody60 μg
TAS013-C02Anti-SARS-CoV-2 Antibody (Control, IgM)10 μg
TAS013-C03HRP-SARS-CoV-2 S protein RBD10 μg
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for IgM titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard capture-ELISA format, providing a rapid detection of anti-SARS-CoV-2 antibody, IgM in serum by SARS-CoV-2 Spike RBD protein. The kit consists of HPR-Spike RBD protein, an Anti-SARS-CoV-2 Antibody (Control, IgM), an anti-Human IgM antobody.

    Your experiment will include 5 simple steps:

    a) Coat the plate with Anti-human IgM Antibody.

    b) After washing and blocking, add your sample to the plate, take the SARS-CoV-2 antobody, IgM as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted HRP-SARS-CoV-2 Spike RBD protein to the plate.

    d) Wash the plate and add TMB or other colorimetric HRP substrate.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 651 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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Price(USD) : $420.00

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