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Your Position: Home > Recombinant Antibodies > Nucleocapsid protein > NUN-S57

Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS57) (Trehalose free)

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Nucleocapsid protein F(ab')2.
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    The cross-reactivity with other coronaviruses has not been tested yet.
  • Application
    This antibody can be paired with other Anti-SARS-CoV-2 Nucleocapsid antibodies to detect SARS-CoV-2 Nucleocapsid protein in sandwich ELISA or LFA assay.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of PBS, pH7.4 .

  • Storage

    For long term storage, the product should be stored in liquid state at  2-6°C upon receipt.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at 2-6°C upon receipt.
    2. The product is validated to be stable after storage at 4°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with blue ice, please inquire the shipping cost.
SDS-PAGE
Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S57) SDS-PAGE gel

Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S57) ELISA bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No.NUN-S57) with a linear range of 0.04-1.25 ng/mL (QC tested).

Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S57) ELISA bioactivity

Detection SARS-CoV-2 Nucleocapsid Protein by Sandwich ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody (Cat. No. NUN-S57) at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein. And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody (Cat. No. NUN-S47) at 1:15000. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (Routinely tested).

  • Background
    Nucleocapsid protein is a most abundant protein of coronavirus. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we identified as the viral nucleocapsid (N) protein. Novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein. The array of diverse functional activities accommodated in the hantaviral N protein goes far beyond to be a static structural protein and makes it an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $380.00

Price(USD) : $2500.00

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