This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.2 kDa. The protein migrates as 10 kDa and 14-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Cynomolgus CD3 epsilon, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Cynomolgus CD3E, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.981 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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