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Your Position: Home > Cytokines > Fas Ligand > Human Fas Ligand / TNFSF6 Protein, His Tag

Human Fas Ligand / TNFSF6 Protein, His Tag

  • Synonym
    FASLG,ALPS1B,APT1LG1,CD178,CD95L,FASL,TNFSF6
  • Source
    Human Fas Ligand, His Tag (FAL-H5241) is expressed from human 293 cells (HEK293). It contains AA Pro 134 - Leu 281 (Accession # AAH17502.1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Pro 134 - Leu 281) AAH17502.1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 17.7 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Fas Ligand, His Tag (Cat. No. FAL-H5241) SDS-PAGE gel

Human Fas Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Fas Ligand, His TagHuman Fas Ligand, His Tag (Cat. No. FAL-H5241) ELISA bioactivity

Immobilized Human Fas Ligand, His Tag (Cat. No. FAL-H5241) at 2 μg/mL (100 μL/well) can bind Human Fas, Fc Tag (Cat. No. FAS-H5252) with a linear range of 2-31 ng/mL (QC tested).

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