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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.8 kDa. The protein migrates as 23-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CTLA-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CTLA-4, His Tag (Cat. No. CT4-H52H9) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H52H9) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Human CTLA-4, His Tag (Cat. No.CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).
Loaded Human B7-1, Fc Tag (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human CTLA-4, His Tag (Cat. No. CT4-H52H9) with an affinity constant of 0.498 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human B7-2, Fc Tag (Cat. No. CD6-H5257) on Protein A Biosensor, can bind Human CTLA-4, His Tag (Cat. No. CT4-H52H9) with an affinity constant of 1.03 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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