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Your Position: Home > Protein-coupled magnetic beads > S protein > MBS-K015

SARS-CoV-2 Spike trimer protein-coupled magnetic beads

This product is still under development. Please contact us if you have interest in this product. We will accelerate the development process accordingly and reserve this product for you as request.
Materials Provided
ItemsSize (2 mg Beads)Size (10 mg Beads)
Pack Size2 mg10 mg
Particle size2 μm2 μm
FormulationPBS, 0.05% Tween-20, pH7.4, with 10% TrehalosePBS, 0.05% Tween-20, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1mg beads/mL)10 mL ultrapure water (1mg beads/mL)
Product FormatPowderPowder
  • Background
    The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike trimer protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. The SARS-CoV-2 Spike trimer protein, His Tag, Super stable trimer (SPN-C52H9) is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This very first SARS-CoV-2 Spike trimer protein-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the Beads at -20℃ for 1 year in lyophilized state.

    Once the Beads reconstitution, please use it immediately.

    Do not to freeze thaw the Beads after reconstitution.
  • Assay Principles
    The Antigen pre-coupled magnetic beads are coupled with biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 Spike trimer protein-coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as immunocapture, biopanning and flow cytometry.

    Application Method:

    a) Reconstitute the Beads following the CoA, wash the Beads and suspended to a certain concentration by adding assay Buffer.

    b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.

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Order Details

Price(USD) : $1300.00

Price(USD) : $3990.00

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Datasheet & Documentation

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DMF (Drug Master File)

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