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Your Position: Home > Isotype Controls > IgG1 Fc > IG1-M8211

Biotinylated Mouse IgG1 Fc protein, Avitag™

  • Synonym
    IgG1
  • Source
    Biotinylated Mouse IgG1 Fc, Avitag™ (IG1-M8211) is expressed from human 293 cells (HEK293). It contains AA Val 98 - Lys 324 (Accession # AAK53870.1).
    Predicted N-terminus: Val 98
  • Molecular Characterization
    Online(Val 98 - Lys 324) AAK53870.1

    This protein carries an Avi tag at the C-terminus.

    The protein has a calculated MW of 27.4 kDa. The protein migrates as 33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse IgG1 Fc, Avitag™ (Cat. No. IG1-M8211) SDS-PAGE gel

Biotinylated Mouse IgG1 Fc, Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $1380.00

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    Datasheet & Documentation

    Request for DMF
    DMF (Drug Master File)

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