>All Other Proteins
>Biotinylated Human CD19, Fc Tag, ultra sensitivity (primary amine labeling)
This protein carries a human IgG1 Fc fragment at the C-terminus. The protein has a calculated MW of 56.3 kDa. The protein migrates as 56-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human CD19, Fc Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-Mouse FMC63 MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD19, Fc Tag, primary amine labeling (Cat. No. CD9-H8259) with a linear range of 0.039-0.625 μg/mL (QC tested).
293 cells were transfected with FCM63-scFv and RFP tag. 2x105 of the cells were first incubated with A. Biotinylated protein control. B. Biotinylated Human CD19, Fc Tag, primary amine labeling (Cat. No. CD9-H8259, 10 μg/mL). C. Biotinylated Human CD19, Fc Tag, primary amine labeling (Cat. No. CD9-H8259, 10 μg/mL) and FMC63 (Mouse Anti-CD19 antibody). FITC Streptavidin was used to analyse with FACS. RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human CD19, Fc Tag, primary amine labeling (Cat. No. CD9-H8259) .
Human T cells were lentivirally transduced with anti-CD19 CAR and cultured for 11 days. Eleven days post-transduction, 1e6 cells were stained for the expression of CD3 and anti-CD19 CAR with FITC Anti-human CD3 antibody and Biotinylated Human CD19, Fc Tag, primary amine labeling (Cat. No. CD9-H8259) followed by PE-conjugated streptavidin, respectively. A. Non-transduced T cells were used as a control for gating of CAR expression. (Data are kindly provided by Beijing Bowei Huaen Medical Technology Co. Ltd.)
Authors: B Chen, et al
Journal: Immunotherapy 2019
Application: Flow Cytometry
Authors: MacLeod DT, et al.
Journal: Mol Ther 2017
Application: Flow Cytometry
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Price(USD) : $385.00
Price(USD) : $1518.00
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