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Your Position: Home > Antibody titer assay kits > S1 protein > TAS-K018

Mouse Anti-SARS-CoV-2 antibody IgG Serologic Assay kit

For research use only.
This product is still under development. Please contact us if you have interest in this product. We will accelerate the development process accordingly and reserve this product for you as request.
Materials Provided
IDComponentsSize
TAS018-C01High-bind Plate96 tests
TAS018-C02SARS-CoV-2 Spike protein S130ug
TAS018-C03Anti-SARS-CoV-2 S1 protein Antibody (Control, Mouse IgG )10ug
TAS018-C04HRP-Goat anti-Mouse IgG 10ug
TAS018-C05Blocking / Dilution Buffer60mL
  • Background
    The ongoing SARS-COV-2 pandemics has sickened 3.7 million people around the world, claiming over 1,000,000 lives. To deal with this global public health crisis, unprecedented efforts are being made to study the virus, SARS-CoV-2. At present, there are 193 new crown vaccine projects registered with the World Health Organization are being developed. To support these studies, ACRO has developed Mouse Anti-SARS-CoV-2 antibody IgG Serologic Assay kit.
  • Application
    This kit is developed for serologic test for Mouse IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 S1 protein . The Kit consists of High-bind Plate, S1 protein , an Anti-SARS-CoV-2 Antibody (Control, Mouse IgG1), an HRP-Anti-Goat anti mouse IgG secondary antibody.

    Your experiment will include 6 simple steps:

    a) Coat the plate with SARS-CoV-2 S1 protein .

    b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.

    c) Add your sample to the plate, take the Anti-SARS-CoV-2 S1 protein Antibody ( Mouse IgG1 )as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.

    d) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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Price(USD) : $600.00

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