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Your Position: Home > Anti-idiotypic Antibodies > FMC63 > FM3-S93

Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (recommended for ADA assay) (MALS verified)

  • Source
    Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) is produced from a Rabbit immunized with FMC63 scFv.
  • Isotype
  • Specificity
    Recognizes FMC63 scFv specifically.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 6 months under sterile conditions after reconstitution.
Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) SDS-PAGE gel

Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. ) MALS images

The purity of Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) was more than 90% and the molecular weight of this protein is around 130-145 kDa verified by SEC-MALS.

Rabbit Anti-Mouse FMC63 scFv Polyclonal AntibodyRabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) ELISA bioactivity

Immobilized FMC63 scFv at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) with a linear range of 0.098-0.78 ng/mL (QC tested).

Rabbit Anti-Mouse FMC63 scFv Polyclonal AntibodyRabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) ELISA bioactivity

Immobilized anti-CD19 antibody (Clone: FMC63) at 1 μg/mL, add increasing concentrations of Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) and then add Biotinylated anti-CD19 antibody (Clone: FMC63) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 78 ng/mL (QC tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $320.00

Price(USD) : $2080.00

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