|TAS022-C01||Pre-coated Human ACE2 Microplate||1 plate|
|TAS022-C02||Negative Control||100 μL|
|TAS022-C03||HRP-SARS-CoV-2 Spike RBD||10 μg|
|TAS022-C04||10xWashing Buffer||50 mL|
|TAS022-C05||Dilution Buffer||50 mL|
|TAS022-C06||Substrate Solution||12 mL|
|TAS022-C07||Stop Solution||7 mL|
|TAS022-C08||Positive Control||100 μL|
The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.
It is recommended not to freeze thaw more than 3 times.
The kit analysis includes 5 simple steps:
a) All reagents are warmed to room temperature (20℃-25℃) before use.
b) Series of sample and control antibody dilution are prepared with dilution buffer. Solution of Spike RBD-HRP is prepared with dilution buffer.
c) The sample dilution is added to plate followed by Spike RBD-HRP solution. For semiquantitative analysis, dilution of control neutralizing antibody is add to the plate followed by Spike RBD-HRP solution.
d) After incubation, plates are washed and TMB or other colorimetric HRP substrate is addded.
e) The substrate reaction is stopped by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background noise prior to statistical analysis. The OD Value reflects the amount of protein bound.
Correlation Analysis of TAS-K022 and FDA Approved ELISA Kit.
High correlation with FDA EUA approved ELISA Kit (detection of serum neutralizing antibody after vaccination).
Anti-SARS-CoV-2 Neutralizing Antibody Titer Detection Data Example.
Operate the ositive sample according to the experimental method in the manual and the recommended sample dilution range. After detecting the gradient dilution of the sample, the maximum dilution when it is determined to be positive according to the cut-off value is 160.The neutralizing antibody titer of the positive sample is 160.(Determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading. )
Emerging VoCs, Lambda, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ELISA kits for neutralizing antibody titer assay, binding antibody titer assay, antibody isotype assay, antigen titer assay and inhibitor screening, etc., which can accelerate the research and development of anti-SARS-CoV-2 drugs and vaccines.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
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