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Your Position: Home > 2019-nCoV proteins > Spike RBD > TAS-K022

Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit

For research use only.
Materials Provided
IDComponentsSize
TAS022-C01Pre-coated Human ACE2 Microplate1 plate
TAS022-C02Negative Control100 μL
TAS022-C03HRP-SARS-CoV-2 Spike RBD 10 μg
TAS022-C0410xWashing Buffer 50 mL
TAS022-C05Dilution Buffer50 mL
TAS022-C06Substrate Solution12 mL
TAS022-C07Stop Solution7 mL
TAS022-C08Positive Control100 μL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses continual threat to human health due to rapid transmission worldwide. The Anti SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit to test the protective neutralizing antibody levels in post-vaccination serum or convalescent serum.
  • Application
    This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody which interfere ACE2/S protein RBD interaction.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

    It is recommended not to freeze thaw more than 3 times.

  • Assay Principles
    The Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay kit is a competitive ELISA. Immobilized Human ACE2 protein on the microplate are incubated with samples and Spike RBD-HRP. Presence of anti-SARS-CoV-2 neutralizing antibody in the samples competes with immobilized Human ACE2 protein to bind Spike RBD-HRP. The intensity of assay signal decreases proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

    The kit analysis includes 5 simple steps:

    a) All reagents are warmed to room temperature (20℃-25℃) before use.

    b) Series of sample and control antibody dilution are prepared with dilution buffer. Solution of Spike RBD-HRP is prepared with dilution buffer.

    c) The sample dilution is added to plate followed by Spike RBD-HRP solution. For semiquantitative analysis, dilution of control neutralizing antibody is add to the plate followed by Spike RBD-HRP solution.

    d) After incubation, plates are washed and TMB or other colorimetric HRP substrate is addded.

    e) The substrate reaction is stopped by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background noise prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data
Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay KitAnti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit (Cat. No. TAS-K022) ELISA bioactivity

Correlation Analysis of TAS-K022 and FDA Approved ELISA Kit.
High correlation with FDA EUA approved ELISA Kit (detection of serum neutralizing antibody after vaccination).

Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay KitAnti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit (Cat. No. TAS-K022) ELISA bioactivity

Anti-SARS-CoV-2 Neutralizing Antibody Titer Detection Data Example.
Operate the ositive sample according to the experimental method in the manual and the recommended sample dilution range. After detecting the gradient dilution of the sample, the maximum dilution when it is determined to be positive according to the cut-off value is 160.The neutralizing antibody titer of the positive sample is 160.(Determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading. )

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Price(USD) : $580.00

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