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| ID | Components | Size |
| RAS032-C01 | Pre-coated SARS-CoV-2 Spike RBD (P.1) Microplate | 1 plate |
| RAS032-C02 | Positive Control | 100 μL |
| RAS032-C03 | Negative Control | 100 μL |
| RAS032-C04 | HRP-Anti-Human IgG | 200 μL |
| RAS032-C05 | 10xWashing Buffer | 50 mL |
| RAS032-C06 | Dilution Buffer | 50 mL |
| RAS032-C07 | Substrate Solution | 12 mL |
| RAS032-C08 | Stop Solution | 7 mL |
This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Spike RBD antibody in serum/plasma in vitro. The Spike RBD antigen used in this kit contains K417T, E484K, N501Y mutations found in the Brazilian variant (known as P.1).
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 39 ng/mL (QC tested).
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