|RAS018-C01||Pre-coated with SARS-CoV-2 Spike RBD Microplate||1 plate|
|RAS018-C02||Anti-SARS-CoV-2 Antibody (Control, Mouse IgG )||100 μL|
|RAS018-C03||HRP-Goat anti-Mouse IgG||10 μg|
|RAS018-C04||10 x Washing Buffer||50 mL|
|RAS018-C05||Dilution Buffer||50 mL|
|RAS018-C06||Substrate Solution||12 mL|
|RAS018-C07||Stop Solution||7 mL|
|RAS018-C08||Positive Control||100 μL|
|RAS018-C09||Negative Control||100 μL|
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
After reconstitution, the stock solution should be kept at -70°C.
It is recommended not to freeze thaw more than 3 times.
This product is stable under storage conditions:
12 months in sealed state;
Used within1 months after opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take theAnti-SARS-CoV-2 Antibody (Control, Mouse IgG )as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Mouse Anti-SARS-CoV-2 Antibody IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Mouse Anti-SARS-CoV-2 Antibody IgG in mouse serum. Detection was performed using HRP-Conjugated Anti-mouse IgG secondary antibody with sensitivity of 20 ng/mL (QC tested).
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