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Your Position: Home > IgG kits > Spike RBD > RAS-T018

Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit

For research use only.
IDComponentsSize
RAS018-C01Pre-coated with SARS-CoV-2 Spike RBD Microplate1 plate
RAS018-C02Anti-SARS-CoV-2 Antibody (Control, Mouse IgG )100 μL
RAS018-C03HRP-Goat anti-Mouse IgG 10 μg
RAS018-C0410 x Washing Buffer 50 mL
RAS018-C05Dilution Buffer50 mL
RAS018-C06Substrate Solution12 mL
RAS018-C07Stop Solution7 mL
RAS018-C08Positive Control100 μL
RAS018-C09Negative Control100 μL
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. The SARS-CoV-2 pandemic presents an extraordinary challenge to the lives and health of the global population, resulting in a total of over 200 million diagnosed cases and 4.9 million death (as of October 2021). It also significantly affected the social, economic and environmental domains throughout the world and changed everyone’s life.

    ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response,developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.

  • Application
    This kit is developed for serologic test for Mouse IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike RBD. The Kit consists of Pre-coated with SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, Mouse IgG1), an HRP-Anti-Goat anti mouse IgG secondary antibody.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate, take theAnti-SARS-CoV-2 Antibody (Control, Mouse IgG )as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data Please refer to DS document for The assay protocol.
 Spike RBD ELISA

Detection of Mouse Anti-SARS-CoV-2 Antibody IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Mouse Anti-SARS-CoV-2 Antibody IgG in mouse serum. Detection was performed using HRP-Conjugated Anti-mouse IgG secondary antibody with sensitivity of 20 ng/mL (QC tested).

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Price(USD) : $580.00

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