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Your Position: Home > Kits > Spike RBD > RAS-T017

Anti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay kit

For research use only.
Materials Provided
IDComponentsSize
RAS017-C01Pre-coated with SARS-CoV-2 Spike RBD Microplate1 plate
RAS017-C02Anti-SARS-CoV-2 Antibody (Control, IgG4)10 μg
RAS017-C03HRP-Mouse anti-Human IgG410 μg
RAS017-C0410 x Washing Buffer 50 mL
RAS017-C05Dilution Buffer50 mL
RAS017-C06Substrate Solution12 mL
RAS017-C07Stop Solution7 mL
  • Background
    The ongoing SARS-COV-2 pandemics has sickened 3.7 million people around the world, claiming over 1,000,000 lives. To deal with this global public health crisis, unprecedented efforts are being made to study the virus, SARS-CoV-2. At present, there are 193 new crown vaccine projects registered with the World Health Organization are being developed. To support these studies, ACRO has developed Anti-SARS-CoV-2 subtype antibody Serologic Assay kit including:IgG1, IgG2 IgG3, IgG4.
  • Application
    This kit is developed for serologic test for IgG4 titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    After reconstitution, the stock solution should be kept at -70°C.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within1 months after opening.
  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The Kit consists of Pre-coated with SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, IgG4), an HRP-Anti-Human IgG4 secondary antibody.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG4 to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay kitAnti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay kit (Cat. No. RAS-T017) ELISA bioactivity

Detection of Anti-SARS-CoV-2 Antibody, Human IgG4 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG4 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG4 antibody with sensitivity of 10 ng/mL (QC tested).

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