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Your Position: Home > 2019-nCoV proteins > Spike RBD > RAS-T013

Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike RBD) 

For research use only.
Materials Provided
IDComponentsSize
RAS013-C01Pre-coated Anti-human lgM antibody Microplate1 plate
RAS013-C02Positive Control100 μL
RAS013-C03Negative Control100 μL
RAS013-C04HRP-SARS-CoV-2 Spike RBD10 μg
RAS013-C0510xWashing Buffer 50 mL
RAS013-C06Dilution Buffer50 mL
RAS013-C07Substrate Solution12 mL
RAS013-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for IgM titer of Anti-SARS-CoV-2 antibody in serum in vitro.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard capture-ELISA format, providing a rapid detection of anti-SARS-CoV-2 antibody, IgM in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated Anti-human lgM antibody Microplate, Positive Control, Negative Control, HRP-SARS-CoV-2 Spike RBD andthe related buffer.

    Your experiment will include 5 simple steps:

    a) Coat the plate with Anti-human IgM Antibody.

    b) After washing and blocking, add your sample to the plate, take the SARS-CoV-2 antobody, IgM as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted HRP-SARS-CoV-2 Spike RBD protein to the plate.

    d) Wash the plate and add TMB or other colorimetric HRP substrate.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike RBD) Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike RBD)  (Cat. No. RAS-T013) ELISA bioactivity

Detection of anti-SARS-CoV-2 antibody, IgM titer by capture-ELISA Assay.
Immobilized anti-Human IgM antibody at 5 μg/mL (100 μL/well) can bind anti-SARS-CoV-2 antibody, IgM in 1:10 human serum. Detection was performed using HRP-SARS-CoV-2 Spike RBD with sensitivity of 11 ng/mL (QC tested).

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Price(USD) : $580.00

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