|RAS013-C01||Pre-coated Anti-human lgM antibody Microplate||1 plate|
|RAS013-C02||Positive Control||100 μL|
|RAS013-C03||Negative Control||100 μL|
|RAS013-C04||HRP-SARS-CoV-2 Spike RBD||10 μg|
|RAS013-C05||10xWashing Buffer||50 mL|
|RAS013-C06||Dilution Buffer||50 mL|
|RAS013-C07||Substrate Solution||12 mL|
|RAS013-C08||Stop Solution||7 mL|
Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.
It is recommended not to freeze thaw more than 3 times.
Your experiment will include 5 simple steps:
a) Coat the plate with Anti-human IgM Antibody.
b) After washing and blocking, add your sample to the plate, take the SARS-CoV-2 antobody, IgM as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add a diluted HRP-SARS-CoV-2 Spike RBD protein to the plate.
d) Wash the plate and add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of anti-SARS-CoV-2 antibody, IgM titer by capture-ELISA Assay.
Immobilized anti-Human IgM antibody at 5 μg/mL (100 μL/well) can bind anti-SARS-CoV-2 antibody, IgM in 1:10 human serum. Detection was performed using HRP-SARS-CoV-2 Spike RBD with sensitivity of 11 ng/mL (QC tested).
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High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed a serial of high-quality antigen and antibody reagents, including super stable trimeric S protein, S RBD, S1, S2, N proteins mainly expressed by HEK 293 and paired antibodies with outstanding sensitivity verified by inactivated virus samples.
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