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Your Position: Home > Kits > Spike S1 > RAS-T012

Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike S1) 

For research use only.
Materials Provided
IDComponentsSize
RAS012-C01Pre-coated Anti-human lgM antibody Microplate1 plate
RAS012-C02Positive Control100 μL
RAS012-C03Negative Control100 μL
RAS012-C04HRP-SARS-CoV-2 Spike S110 μg
RAS012-C0510xWashing Buffer 50 mL
RAS012-C06Dilution Buffer50 mL
RAS012-C07Substrate Solution12 mL
RAS012-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for IgM titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Storage

    The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

  • Assay Principles
    This assay kit employs a standard capture-ELISA format, providing a rapid detection of anti-SARS-CoV-2 antibody, IgM in serum by SARS-CoV-2 Spike S1. The kit consists of Pre-coated Anti-human lgM antibody Microplate, Positive Control, Negative Control, HRP-SARS-CoV-2 S1 and the related buffer.

    Your experiment will include 4 simple steps:

    a) The samples and Control are diluted by Dilution Buffer. Add the diluted samples and control to the plate.

    b) Add the diluted HRP-SARS-CoV-2 Spike S1 to the plate.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike S1) Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike S1)  (Cat. No. RAS-T012) ELISA bioactivity

Detection of anti-SARS-CoV-2 antibody, IgM titer by capture-ELISA Assay.
Immobilized anti-Human IgM antibody at 5 μg/mL (100 μL/well) can bind anti-SARS-CoV-2 antibody, IgM in 1:10 human serum. Detection was performed using HRP-SARS-CoV-2 Spike S1 with sensitivity of 39 ng/mL (QC tested).

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Price(USD) : $580.00

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