|RAS009-C01||Pre-coated SARS-CoV-2 Spike RBD Microplate||1 plate|
|RAS009-C02||Positive Control||200 μL|
|RAS009-C03||Negative Control||200 μL|
|RAS009-C04||HRP-SARS-CoV-2 Spike RBD||10 μg|
|RAS009-C05||10xWashing Buffer||50 mL|
|RAS009-C06||Dilution Buffer||50 mL|
|RAS009-C07||Substrate Solution||12 mL|
|RAS009-C08||Stop Solution||7 mL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted HRP-SARS-CoV-2 Spike RBD to the plate. The HRP-SARS-CoV-2 Spike RBD is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody (Spike RBD) titer by sandwich-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 0.5 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Antibody (Spike RBD). Detection was performed using HRP-SARS-CoV-2 Spike RBD with sensitivity of 195 ng/mL (QC tested).
Price(USD) : $580.00
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