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Your Position: Home > 2019-nCoV proteins > Spike RBD > RAS-T009

Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS009-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS009-C02Positive Control200 μL
RAS009-C03Negative Control200 μL
RAS009-C04HRP-SARS-CoV-2 Spike RBD 10 μg
RAS009-C0510xWashing Buffer 50 mL
RAS009-C06Dilution Buffer50 mL
RAS009-C07Substrate Solution12 mL
RAS009-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for Total Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

    It is recommended not to freeze thaw more than 3 times for powder.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of total anti-SARS-CoV-2 antibody in serum by SARS-CoV-2 Spike protein RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Spike RBD and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted HRP-SARS-CoV-2 Spike RBD to the plate. The HRP-SARS-CoV-2 Spike RBD is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD) (Cat. No. RAS-T009) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Antibody (Spike RBD) titer by sandwich-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 0.5 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Antibody (Spike RBD). Detection was performed using HRP-SARS-CoV-2 Spike RBD with sensitivity of 195 ng/mL (QC tested).

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Price(USD) : $580.00

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