|RAS009-C01||Pre-coated SARS-CoV-2 Spike RBD Microplate||1 plate|
|RAS009-C02||Positive Control||200 μL|
|RAS009-C03||Negative Control||200 μL|
|RAS009-C04||HRP-SARS-CoV-2 Spike RBD||10 μg|
|RAS009-C05||10xWashing Buffer||50 mL|
|RAS009-C06||Dilution Buffer||50 mL|
|RAS009-C07||Substrate Solution||12 mL|
|RAS009-C08||Stop Solution||7 mL|
The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.
It is recommended not to freeze thaw more than 3 times for powder.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted HRP-SARS-CoV-2 Spike RBD to the plate. The HRP-SARS-CoV-2 Spike RBD is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody (Spike RBD) titer by sandwich-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 0.5 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Antibody (Spike RBD). Detection was performed using HRP-SARS-CoV-2 Spike RBD with sensitivity of 195 ng/mL (QC tested).
Emerging VoCs, Lambda, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ELISA kits for neutralizing antibody titer assay, binding antibody titer assay, antibody isotype assay, antigen titer assay and inhibitor screening, etc., which can accelerate the research and development of anti-SARS-CoV-2 drugs and vaccines.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
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