Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)

背景（Background）

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.

应用说明（Application）

This kit is developed for serologic test for Total Anti-SARS-CoV-2 antibody in serum/plasma in vitro.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储 & 运输（Storage & Shipping）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of total anti-SARS-CoV-2 antibody in serum by SARS-CoV-2 Spike protein RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Spike RBD and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted HRP-SARS-CoV-2 Spike RBD to the plate. The HRP-SARS-CoV-2 Spike RBD is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.