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Your Position: Home > Kits > Spike RBD > RAS-T009

Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS009-C01HRP-SARS-CoV-2 Spike RBD protein10 μg
RAS009-C02Anti-SARS-CoV-2 Antibody (Control, IgG)10 μg
TAS002-C02SARS-CoV-2 Spike RBD protein10 μg
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for Total Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of total anti-SARS-CoV-2 antibody in serum by SARS-CoV-2 Spike protein RBD. The kit consists of Spike protein RBD, an Anti-SARS-CoV-2 Antibody (Control,IgG), an HRP-Spike protein RBD.

    Your experiment will include 6 simple steps:

    a) Coat the plate with SARS-CoV-2 Spike protein RBD.

    b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.

    c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    d) Add a diluted Secondary antigen HRP-SARS-CoV-2 Spike protein RBD to the plate. The Secondary antigen is diluted by Dilution Buffer.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD) (Cat. No. RAS-T009) ELISA bioactivity

Detection the total SARS-CoV-2 antibody titer by ELISA Assay.
Immobilized SARS-CoV-2 S protein RBD at 0.5 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Antibody, chimeric mAb, in human serum. Detection was performed using HRP-Conjugated SARS-CoV-2 Spike RBD with sensitivity of 8 ng/mL (QC tested).

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