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Your Position: Home > 2019-nCoV proteins > Nucleocapsid protein > RAS-T008

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Nucleocapsid Protein)

For research use only.
Materials Provided
IDComponentsSize
RAS008-C01Pre-coated SARS-CoV-2 Nucleocapsid Protein Microplate1 plate
RAS008-C02Positive Control100 μL
RAS008-C03Negative Control100 μL
RAS008-C04HRP-Anti-Human IgG200 μL
RAS008-C0510 x Washing Buffer 50 mL
RAS008-C06Dilution Buffer50 mL
RAS008-C07Substrate Solution12 mL
RAS008-C08Stop Solution7 mL
  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Application
    This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Nucleocapsid Antibody in serum/plasma in vitro.
    It is for research use only.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Nucleocapsid Protein. The Kit consists of Pre-coated SARS-CoV-2 Nucleocapsid Protein Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Nucleocapsid Protein)Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Nucleocapsid Protein) (Cat. No. RAS-T008) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Nucleocapsid Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 0.24 ng/mL (QC tested).

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Price(USD) : $580.00

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