|RAS008-C01||Pre-coated SARS-CoV-2 Nucleocapsid Protein Microplate||1 plate|
|RAS008-C02||Positive Control||100 μL|
|RAS008-C03||Negative Control||100 μL|
|RAS008-C04||HRP-Anti-Human IgG||200 μL|
|RAS008-C05||10 x Washing Buffer||50 mL|
|RAS008-C06||Dilution Buffer||50 mL|
|RAS008-C07||Substrate Solution||12 mL|
|RAS008-C08||Stop Solution||7 mL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Nucleocapsid Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 0.24 ng/mL (QC tested).
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