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Your Position: Home > 2019-nCoV proteins > Nucleocapsid protein > RAS-A010

SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit

For research use only.
Materials Provided
RAS010-C01Pre-coated Anti-SARS-CoV-2 Nucleocapsid Antibody Microplate1 plate
RAS010-C02SARS-CoV-2 Nucleocapsid Protein10 ug
RAS010-C03Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody100 μL
RAS010-C04Streptavidin-HRP100 μL
RAS010-C0510xWashing Buffer 50 mL
RAS010-C06Dilution Buffer50 mL
RAS010-C07Substrate Solution12 mL
RAS010-C08Stop Solution7 mL
  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Application
    This kit is developed for detecting the SARS-CoV-2 Nucleocapsid Protein in cell supernatant or SARS-CoV-2 virus in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Nucleocapsid Protein. The kit consists of Anti-SARS-CoV-2 Nucleocapsid Antibody, an SARS-CoV-2 Nucleocapsid Protein as Control, an biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody and HRP-Streptavidin.

    Your experiment will include 6 simple steps:

    a) Coat the plate with Anti-SARS-CoV-2 Nucleocapsid Antibody.

    b) After washing and blocking, add your sample to the plate, take the SARS-CoV-2 Nucleocapsid protein as Control sample. The samples and Control sample are diluted by Lysis Buffer.

    c) Add a diluted Secondary antibody biotin-anti-SARS-CoV-2 Nucleocapsid Antibody to the plate. The Secondary antody is diluted by Dilution Buffer.

    d) Add a diluted Streptavidin-HRP to the plate.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
SARS-CoV-2 Nucleocapsid Protein Titer Assay KitSARS-CoV-2 Nucleocapsid Protein Titer Assay Kit (Cat. No. RAS-A010) ELISA bioactivity

Detection of Monoclonal SARS-CoV-2 Nucleocapsid Protein titer by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Protein Antibody at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein and Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody. Detection was performed using Streptavidin-HRP with sensitivity of 12.5 pg/mL (QC tested).

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Price(USD) : $580.00

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