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Your Position: Home > Kits > Spike S1 > RAS-T001

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike S1)

For research use only.
Materials Provided
IDComponentsSize
RAS001-C01High-bind Plate1 plate
RAS001-C02SARS-CoV-2 Spike S130 μg
RAS001-C03Anti-SARS-CoV-2 Antibody (Control, IgG)10 μg
RAS001-C04HRP-Anti-Human IgG10 μg
RAS001-C05Blocking / Dilution Buffer60 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike protein S1. The kit consists of High-bind Plate, Spike protein S1, an Anti-SARS-CoV-2 Antibody (Control,IgG), an HRP-Anti-Human IgG secondary antibody and Blocking/Dilution buffer.

    Your experiment will include 6 simple steps:

    a) Coat the plate with SARS-CoV-2 Spike protein S1.

    b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.

    c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.

    d) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike S1)Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike S1) (Cat. No. RAS-T001) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 S1 protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 48 ng/mL (QC tested).

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