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Your Position: Home > All Other Proteins > ACE2 > AC2-H82E8

Biotinylated Monomeric Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)

  • Synonym
    ACE-2,ACEH,ACE2
  • Source
    Biotinylated Monomeric Human ACE2, His,Avitag (AC2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 611 (Accession # Q9BYF1-1).
    Predicted N-terminus: Gln 18
  • Molecular Characterization
    Online(Gln 18 - Ser 611) Q9BYF1-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 72.3 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Monomeric Human ACE2, His,Avitag (Cat. No. AC2-H82E8) SDS-PAGE gel

Biotinylated Monomeric Human ACE2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Monomeric Human ACE2, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Monomeric Human ACE2, His,Avitag (Cat. No. AC2-H82E8) was more than 90% and the molecular weight of this protein is around 75-90 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Monomeric Human ACE2, His,AvitagBiotinylated Monomeric Human ACE2, His,Avitag (Cat. No. AC2-H82E8) ELISA bioactivity

Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E8) with a linear range of 0.039-0.625 μg/mL (QC tested).

  • Background
    Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $380.00

Price(USD) : $1570.00

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