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Your Position: Home > Cytokines > TNF-alpha > Human TNF-alpha Biotinylated Protein

Biotinylated Human TNF-alpha, epitope tag free, ultra sensitivity (primary amine labeling)

  • Synonym
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • Source
    MABSol® Biotinylated Human TNF-alpha (TNA-H8211) is expressed from human HEK293 cells. It contains AA Val 77 - Leu 233 (Accession # NP_000585.2). It is the biotinylated form of ActiveMax® Recombinant Human TNF-alpha (Cat # TNA-H4211).
    Predicted N-terminus: Val 77
  • Molecular Characterization
    Online(Val 77 - Leu 233) NP_000585.2

    The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1-3 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human TNF-alpha, Tag Free (Cat. No. TNA-H8211) SDS-PAGE gel

Biotinylated Human TNF-alpha, Tag Free on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human TNF-alpha, Tag FreeBiotinylated Human TNF-alpha, Tag Free (Cat. No. TNA-H8211) ELISA bioactivity

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, Tag Free (Cat. No. TNA-H8211) with a linear range of 0.4-6 ng/mL (QC tested).

Biotinylated Human TNF-alpha, Tag FreeBiotinylated Human TNF-alpha, Tag Free (Cat. No. TNA-H8211) ELISA bioactivity

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 50 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, Tag Free (Cat. No. TNA-H8211) with a linear range of 0.4-6 ng/mL (Routinely tested).

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TNF-alpha Products

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  • Lastest Research Phase:Approved
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