The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Biotinylated Human TNF-alpha on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, Tag Free (Cat. No. TNA-H8211) with a linear range of 0.4-6 ng/mL (QC tested).
Quantification of active infliximab in human serum with liquid chromatography-tandem mass spectrometry using a tumor necrosis factor alpha -based pre-analytical sample purification and a stable isotopic labeled infliximab bio-similar as internal standard:
Authors: El Amrani M, et al.
Journal: J Chromatogr A 2016
Authors: P Sabhachandani, et al.
Journal: Microchim Acta 2015
Application: Microsphere-based assay
Authors: B Yu, et al.
Journal: US20140155285A1 2014
Application: Antibody Screening by FACS
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $290.00
Price(USD) : $1250.00
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