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HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (SPN-H52H5) is the ectodomain of HCoV-HKU1 (isolate N5) spike protein which contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. The S1/S2 furin-recognition site 752-RRKRR-756 is mutated to GGSGS to generate the uncleaved protein. Proline substitutions (N1067P, L1068P) are introduced to stabilize the trimeric prefusion state of the spike protein.
    Predicted N-terminus: Ala 13
  • Molecular Characterization
    Online(Ala 13 - Asn 1276) Q0ZME7-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 145.5 kDa. The protein migrates as 180-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (Cat. No. SPN-H52H5) SDS-PAGE gel

HCoV-HKU1 (isolate N5) Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (Cat. No. ) MALS images

The purity of HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (Cat. No. SPN-H52H5) was more than 85% and the molecular weight of this protein is around 600-660 kDa verified by SEC-MALS.

  • Background
    Human coronavirus HKU1 (HCoV-HKU1) is one of seven known coronaviruses to infect humans. The virus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the N-acetyl-9-O-acetylneuraminic acid receptor. It causes an upper respiratory disease with symptoms of the common cold, but can advance to pneumonia and bronchiolitis. The spike protein of HCoV-HKU1 is a trimer that contains two main subunits: the S1 subunit is responsible for receptor binding, while the S2 subunit mediates membrane fusion.
  • Research Progress of Spike protein
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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