Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > EGF > EGF-H52H3

ActiveMax® Human EGF Protein, His Tag

  • Synonym
    EGF,URG,HOMG4
  • Source
    ActiveMax® Human EGF, His Tag(EGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1 ).
  • Molecular Characterization
    EGF Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
EGF SDS-PAGE

ActiveMax® Human EGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-Organoid culture
 EGFOrganoid culture

Human EGF (Cat. EGF-H52H3), Noggin (Cat. NON-H5257) and R-spondin1 (Cat. RS6-H4220) could support the growth of mouse intestinal organoids.

Bioactivity-ELISA
 EGF ELISA

Immobilized Human EGF, His Tag (Cat. No. EGF-H52H3) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-5 ng/mL (QC tested).

Bioactivity-Bioactivity CELL BASE
 EGF CELL

Response to human EGF protein (Fold).
The EGFR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein (AcroBiosystems, Cat. No. EGF-H52H3). The max induction fold was approximately 56 (Routinely tested).

  • Background
    Human epidermal growth factor (EGF) is also known as HOMG4 and  URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $62.00

Price(USD) : $158.00

Recommended Products
Monoclonal Anti-His Tag Antibody

Price(USD) : $240.00

Price(USD) : $1580.00

HRP conjugated Anti-His Tag Antibody

Price(USD) : $250.00

Price(USD) : $1680.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message