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Your Position: Home > > CD3 epsilon > CDE-M525a

Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag™ (Clone: OKT3), premium grade

  • Source
    Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3), premium grade is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with CD3 epsilon.
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Isotype
    Mouse IgG2a/kappa
  • Specificity
    This product is a specific antibody specifically reacts with CD3 epsilon.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Endotoxin
    Less than 0.002 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at liquid state at -70°C.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 2-8°C for 12 months under sterile condition;
    2. -70°C for 24 months.
SDS-PAGE
CD3 epsilon SDS-PAGE

Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3), premium grade on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
CD3 epsilon MALS images

The purity of Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3), premium grade (Cat. No. CDE-M525a) is more than 95% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Bioactivity-Elisa
 CD3 epsilon ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3) (Cat. No. CDE-M525a) with a linear range of 2-16 ng/mL (QC tested).

Bioactivity-FACS
 CD3 epsilon FACS

5e5 of Jurkat cells were stained with 100 μL of 1 μg/mL of Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3), premium grade (Cat. No. CDE-M525a) and isotype control antibody respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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