>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Trastuzumab Antibodies on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1b) with a linear range of 0.4-6 ng/mL (QC tested).
Immobilized Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1b) at 2 μg/mL, add increasing concentrations of Trastuzumab in the 10% human serum and then add Biotinylated Anti-Trastuzumab Antibodies (recommended for PK/PD) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 48 ng/mL (QC tested).
Serial dilutions of Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 4.58 μg/mL (Routinely tested).
Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1b) with an affinity constant of 0.729 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $300.00
Price(USD) : $1980.00
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