rh MMP1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 52.7 kDa. The predicted N-terminus is Phe 20. The reducing (R) protein migrates as 52-55 kDa in SDS-PAGE .
Pre-activation is required for enzymatic assays. Please dilute Human MMP-1 to 100 µg/mL in TCNB buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5), and then add p-aminophenylmercuric acetate (APMA, Sigma, Catalog # A-9563) to a final concentration of 1 mM. Please keep the enzyme with APMA for 1 hour at 37°C. *100mM APMA stock solution should be prepared in DMSO. Please avoid adding high concentration APMA solution (>20mM) directly into the reaction as it tends to precipitate. A pre-dilution step is highly recommended.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, 10 mM CaCl2, pH6.0 with 0.05% Brij-35. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human MMP-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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