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Your Position: Home > Fc Receptor Proteins > Fc gamma RIIIA / CD16a > Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI verified)

Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI verified)

  • Synonym
    FCGR3A,CD16A,FCG3,FCGR3,IGFR3
  • Source
    Human CD16a (F176), His Tag (SPR & BLI verified) (CDA-H5220) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).
    Predicted N-terminus: Gly 17
  • Molecular Characterization
    Online(Gly 17 - Gln 208) P08637-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.7 kDa. The protein migrates as 39-51 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) SDS-PAGE gel

Human CD16a (F176), His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) on CM5 Chip via anti-His antibody, can bind  Rituximab with an affinity constant of 0.63 μM as determined in a SPR assay (Biacore T200) (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) with an affinity constant of 0.51 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $1800.00

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