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Your Position: Home > Bio-Markers & CD Antigens > Mouse PCSK9 Protein

Mouse PCSK9 Protein

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Mouse PCSK9, His Tag (PC9-M5228) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1).
    Predicted N-terminus: Gln 35
  • Molecular Characterization
    PCSK9(Gln 35 - Gln 694) AAH38085.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 72 kDa. The protein migrates as 20 kDa and 64 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >97% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse PCSK9, His Tag (Cat. No. PC9-M5228) SDS-PAGE gel

Mouse PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Bioactivity
Mouse PCSK9, His TagMouse PCSK9, His Tag (Cat. No. PC9-M5228) ELISA bioactivity

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 10 μg/mL (100 μl/well) can bind Mouse PCSK9, His Tag (Cat. No. PC9-M5228 ) with a linear range of 2-30 ng/mL (QC tested).

  • Background
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $280.00

Inventory : In stock

Price(USD) : $1980.00

Inventory : In stock

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