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Your Position: Home > Viral Proteins > Spike protein > SPN-C52Ht

SARS-CoV-2 Spike Trimer (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His Tag (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Ht) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Mutations T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N were identified in the SARS-CoV-2 Delta sub-lineages (Pango lineage: AY.1/AY.2/AY.3; also known as "Delta Plus"). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
    Predicted N-terminus: Val 16
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 137.8 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 Spike Trimer, His Tag  (Cat. No. SPN-C52Ht) SDS-PAGE gel

SARS-CoV-2 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
SARS-CoV-2 Spike Trimer, His Tag  (Cat. No. ) MALS images

The purity of SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Ht) was more than 85% and the molecular weight of this protein is around 510-564 kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS-CoV-2 Spike Trimer, His Tag SARS-CoV-2 Spike Trimer, His Tag  (Cat. No. SPN-C52Ht) ELISA bioactivity

Immobilized SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Ht) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122)) with a linear range of 0.1-3 ng/mL (QC tested).

SARS-CoV-2 Spike Trimer, His Tag SARS-CoV-2 Spike Trimer, His Tag  (Cat. No. SPN-C52Ht) ELISA bioactivity

Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52H) with a linear range of 0.1-3 ng/mL (Routinely tested).

SARS-CoV-2 Spike Trimer, His Tag SARS-CoV-2 Spike Trimer, His Tag  (Cat. No. SPN-C52Ht) ELISA bioactivity

Immobilized SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Ht) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.8-13 ng/mL (Routinely tested).

SARS-CoV-2 Spike Trimer, His Tag SARS-CoV-2 Spike Trimer, His Tag  (Cat. No. SPN-C52Ht) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Ht) with a linear range of 0.2-13 ng/mL (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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