This HEK293 cell line is engineered to stably overexpress human angiotensin I converting enzyme 2 (ACE2) and is highly permissive to infection by lentiviral particles pseudotyped with SARS-COV-2 spike protein. The HEK293/Human ACE2 Overexpression Stable Cell Line is a powerful tool for in vitro screening and characterization of potential drugs against SARS-COV-2.
Screening of small molecule inhibitors and/or neutralizing antibodies of the ACE2‑Spike interaction
Adherent, 1:5~1:10 every 2~3 days.
90% DMEM high glucose +10% FBS
10% FBS+10% DMSO+80% DMEM high glucose
1 vial contains ~ 1x10^6 cells in 1 ml of 10% DMSO in (DMEM+10%FBS).
Frozen in liquid nitrogen
See data sheet for detailed culturing and assay protocol
Flow Cytometry assay shows that SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) can bind to HEK293/Human ACE2 Overexpression Stable Cell Line (Cat. No. CHEK-ATP042). The concentration of S1 used is 20 μg/mL (QC tested).
Emerging VoCs, Lambda, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ELISA kits for neutralizing antibody titer assay, binding antibody titer assay, antibody isotype assay, antigen titer assay and inhibitor screening, etc., which can accelerate the research and development of anti-SARS-CoV-2 drugs and vaccines.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
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