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HEK293/Human ACE2 Overexpression Stable Cell Line

  • Description

    This HEK293 cell line is engineered to stably overexpress human angiotensin I converting enzyme 2 (ACE2) and is highly permissive to infection by lentiviral particles pseudotyped with SARS-COV-2 spike protein. The HEK293/Human ACE2 Overexpression Stable Cell Line is a powerful tool for in vitro screening and characterization of potential drugs against SARS-COV-2.

  • Application

    Screening of small molecule inhibitors and/or neutralizing antibodies of the ACE2‑Spike interaction

  • Growth Properties

    Adherent, 1:5~1:10 every 2~3 days.

  • Culture Medium

    90% DMEM high glucose +10% FBS

  • Freeze Medium

    10% FBS+10% DMSO+80% DMEM high glucose

  • Format/Supplied As

    1 vial contains ~ 1x10^6 cells in 1 ml of 10% DMSO in (DMEM+10%FBS).

  • Storage

    Frozen in liquid nitrogen

  • Instructions for Use

    See data sheet for detailed culturing and assay protocol

FACS assay shows that recombinant Biotinylated FACS

Flow Cytometry assay shows that SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) can bind to HEK293/Human ACE2 Overexpression Stable Cell Line (Cat. No. CHEK-ATP042). The concentration of S1 used is 20 μg/mL (QC tested).

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Price(USD) : $2380.00

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