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Your Position: Home > Cytokines > Complement component 3 > CO3-M52H4

Mouse Complement component 3 / Complement C3 Protein, His Tag (MALS verified)

  • Synonym
    Complement C3,CPAMD1
  • Source
    Mouse Complement component 3, His Tag (CO3-M52H4) is expressed from human 293 cells (HEK293). It contains AA Ile 25 - Asn 1663 (Accession # P01027-1).
    Predicted N-terminus: Ile 25
  • Molecular Characterization
    Online(Ile 25 - Asn 1663) P01027-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 186.1 kDa. The protein migrates as 70 kDa, 100-120 kDa and 160-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse Complement component 3, His Tag (Cat. No. CO3-M52H4) SDS-PAGE gel

Mouse Complement component 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Mouse Complement component 3, His Tag (Cat. No. ) MALS images

The purity of Mouse Complement component 3, His Tag (Cat. No. CO3-M52H4) is more than 90% and the molecular weight of this protein is around 167-204 kDa verified by SEC-MALS.

  • Background
    C3 is the major complement component serum. It is mainly synthesized by macrophages and liver. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. It is cleaved into two fragments, C3a and C3b. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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