Human ADAM12, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 55.2 kDa (Propeptide 20.1 kDa and mature form 34.5 kDa). Human ADAM12, His Tag was cleaved into the propeptide and the mature form. The reducing (R) protein migrates as 65 kDa (Propeptide 23 kDa and mature form 46 kDa) in SDS-PAGE due to glycosylation, corresponding to the proprotein form, the mature form and the propeptide respectively.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 . Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human ADAM12, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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