rh ADAM12 is fused with 6 ×His tag at the C terminus, and has a calculated MW of 55.2 kDa (Arg 29 - Asp 513, Propeptide 20.1 kDa + mature form 34.5 kDa). The rh ADAM12 (Arg 29 - Asp 513) was cleaved into the propeptide and the mature form. The reducing (R) protein migrates as 65 kDa, 46 kDa and 23 kDa in SDS-PAGE due to glycosylation, corresponding to the proprotein form, the mature form and the propeptide respectively.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 . Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human ADAM12, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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