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Your Position: Home > All Other Proteins > SpeB > SPB-S5115

SpeB Protease

  • Synonym
    Streptopain,Exotoxin type B,Streptococcal cysteine proteinase
  • Source
    SpeB  is a unique recombinantly produced cysteine protease that digests antibodies from many species and subclasses in the hinge region, producing a homogenous pool of Fab fragments. The enzyme requires reducing conditions for activity on IgG. Human, mouse, rat, goat and sheep IgG are digested by SpeB, yielding Fab and Fc fragments. The primary digestion site on human IgG1 is ..KTHT / CPPCPAPE... The molecular weight of SpeB is 28 kDa and the enzyme is tag free. It requires a reducing agent in the digestion reaction and the reducing agent is likely to reduce interchain thiols.
    Predicted N-terminus: Gln 146
  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 27.6 kDa.

  • Application
    Purified and homogenous Fab fragments can be used in many applications, including: 1. Study monovalent binding of Fab fragments to the antigen. 2. Elimination of Fc-mediated effector functions. 3. Reduced unspecific binding from Fc interactions
    It digests IgG in commonly used buffers with pH ranging from 6.5 to 8.0 and best activity is obtained at 37°C. The enzyme requires reducing agent in the digestion reaction and depending on the reducing conditions, there is a chance that the interchain thiols will be reduced. One unit digests ≥ 95% of 1 µg human IgG1 when incubated in PBS with 5 mM DTT or TCEP, pH 7.4 at 37°C for 1 hour. The purity of the protein is greater than 90%.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, 1 mM TCEP, pH7.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.

  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
  • Background
    SpeB is derived from Streptococcus pyogenes. It is an important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. It also cleaves human IL1B precursor to form biologically active IL1B and can induce apoptosis in human monocytes and epithelial cells in vitro, reduces phagocytic activity in monocytic cells. Thus, it may play a role in bacterial colonization, invasion, and inhibition of wound healing.
  • Clinical and Translational Updates

      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $298.00

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