Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > CAR-T > FMC63 > FM3-Y45A1

Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Carrier-free) (recommended for ADA assay) DMF Filed

  • Source
    Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with FMC63 scFv.
  • Isotype
    Mouse IgG1 / kappa
  • Specificity
    Specifically recognizes the antigen-recognition domain of FMC63 derived antibody
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 6 months under sterile conditions after reconstitution.
SDS-PAGE
Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free) (Cat. No. FM3-Y45A1) SDS-PAGE gel

Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free) (Cat. No. ) MALS images

The purity of Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free) (Cat. No. FM3-Y45A1) is more than 95% and the molecular weight of this protein is around 140-160kDa verified by SEC-MALS.

Bioactivity-ELISA
Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free)Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free) (Cat. No. FM3-Y45A1) ELISA bioactivity

Immobilized FMC63 scFv, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Carrier-free) (Cat. No. FM3-Y45A1) with a linear range of 1-19 ng/mL. Anti-DNP antibody, mouse IgG1 (Cat. No. DNP-M1) w) was used as an isotype control (QC tested).

Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free)Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Carrier-free) (Cat. No. FM3-Y45A1) ELISA bioactivity

ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag was inhibited by increasing concentration of Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Carrier-free) (Cat. No. FM3-Y45A1). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Carrier-free)(Cat. No. FM3-Y45A1) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay (Routinely tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : Inquire

Price(USD) : Inquire

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message