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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-3 Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human IL-3 Protein, His Tag, premium grade (Cat. No. IL3-H52H9) is more than 85% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Immobilized Human IL-3 Protein, His Tag, premium grade (Cat. No. IL3-H52H9) at 5 μg/mL (100 μL/well) can bind Human IL-3 R alpha Protein, Llama IgG2b Fc Tag (Cat. No. ILA-H5255) with a linear range of 0.039-2.5 μg/mL (QC tested).
Loaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. IL3-H52H9) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) on SA Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. IL3-H52H9) with an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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