This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.3 kDa. The protein migrates as 53-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD30, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CD30, His Tag (Cat. No. CD0-H5229) was more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Immobilized Human CD30, His Tag (Cat. No. CD0-H5229) at 2 μg/mL (100 μL/well) can bind Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) with a linear range of 0.8-25 ng/mL (QC tested).
Loaded Anti-CD30 MAb, Human IgG1 on Protein A Biosensor, can bind Human CD30, His Tag (Cat. No. CD0-H5229) with an affinity constant of 10.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) on Protein A Biosensor, can bind Human CD30, His Tag (Cat. No. CD0-H5229) with an affinity constant of 70.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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