This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.3 kDa. The protein migrates as 48-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human CD30, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-CD30 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human CD30, His Tag (Cat. No. CD0-H5229) with a linear range of 1-15.6 ng/mL (Routinely tested).
Loaded Anti-CD30 MAb, Human IgG1 on Protein A Biosensor, can bind Human CD30, His Tag (Cat. No. CD0-H5229) with an affinity constant of 16.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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