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Your Position: Home > All Other Proteins > CD30 > Human CD30 /TNFRSF8 Protein

Human CD30 / TNFRSF8 Protein

  • Synonym
    TNFRSF8,CD30,D1S166E,Ki-1
  • Source
    Human CD30, His Tag (CD0-H5229) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    CD30(Phe 19 - Lys 379) NP_001234.2

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.3 kDa. The protein migrates as 48-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CD30, His Tag (Cat. No. CD0-H5229) SDS-PAGE gel

Human CD30, His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human CD30, His TagHuman CD30, His Tag (Cat. No. CD0-H5229) ELISA bioactivity

Immobilized Anti-CD30 MAb, Human IgG1 at 2μg/mL (100 μL/well) can bind Human CD30, His Tag (Cat. No. CD0-H5229) with a linear range of 1-15.6 ng/mL (Routinely tested).

Human CD30, His TagHuman CD30, His Tag (Cat. No. CD0-H5229) ELISA bioactivity

Immobilized Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) at 5 μg/mL (100 μL/well) can bind Human CD30, His Tag (Cat. No. CD0-H5229) with a linear range of 8-125 ng/mL (QC tested).

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