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Your Position: Home > Enzyme > Endo H > ENH-S5116

Endoglycosidase H (Endo H)

  • Synonym
    Endo H, EndobetaNacetylglucosaminidase H,DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase H,Endoglycosidase H,Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase H
  • Source
    Endoglycosidase H (Endo H) is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. Endo H cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from asparagine-linked glycoproteins.The molecular weight of Endo H is 29 kDa and the enzyme is tag free. The purity of the protein is greater than 90% by HPLC.
  • Molecular Characterization

    This protein carries no "tag"

    The protein has a calculated MW of 28.9 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE).

  • Application
    One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10µl.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 50 mM NaCl, 5 mM EDTA, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 6 months under sterile conditions.
SDS-PAGE
Endo H SDS-PAGE

Endo H on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins. It is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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