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Your Position: Home > Protein > Endo H > ENH-S5116

Endo H (500U/μl)

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  • Product Details
    Endo H is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. It cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from asparagine-linked glycoproteins.
  • Application
    1. Endo H cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.
  • Unit Definition
    One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10µl.
  • Quility Control
    Less than 1.0 EU per μg by the LAL method. >95% as determined by SDS-PAGE.
  • Purity
    >95% as determined by SDS-PAGE.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 50 mM NaCl, 5 mM EDTA, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 6 months under sterile conditions.
Bioactivity
 Endo H ENZYME

One unit remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl.

  • Clinical and Translational Updates

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ETD of in-stock products: 2 business days

Price(USD) : $35.00

Price(USD) : $135.00

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