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Your Position: Home > All Other Proteins > Cathepsin B > Human Cathepsin B / CTSB Protein, His Tag

Human Cathepsin B / CTSB Protein, His Tag

  • Synonym
    CTSB,CPSB,APPS
  • Source
    Human Cathepsin B, His Tag (CTB-H5222) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # NP_001899).
    Predicted N-terminus: Arg18 (pro-form) or Phe74 (mature-form)
  • Molecular Characterization
    Online(Arg 18 - Ile 339) NP_001899

    Human Cathepsin B, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.7 kDa (pro-form) and 29.0 kDa (mature-form). Due to glycosylation, The reducing (R) protein migrates as 42-50 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Cathepsin B, His Tag (Cat. No. CTB-H5222) SDS-PAGE gel

Human Cathepsin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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