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Your Position: Home > Protein > Glycoprotein D (HSV-2) > GLD-V52H4

HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (MALS verified)

  • Synonym
    gD
  • Source
    HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (GLD-V52H4) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Tyr 310 (Accession # P03172-1).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    Online(Lys 26 - Tyr 310) P03172-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 33.7 kDa. The protein migrates as 44-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H4) SDS-PAGE gel

HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (Cat. No. ) MALS images

The purity of HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H4) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His TagHSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H4) ELISA bioactivity

Immobilized HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H4) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with a linear range of 0.2-5 ng/mL (QC tested).

  • Background
    Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein D (gD) is a structural component of the herpes simplex virus type 1 (HSV-1) envelope which is essential for virus entry and fusion with host cells. gD plays an important role by binding to the host receptors such as herpes virus entry mediator (HVEM) and nectin-1, a member of the immunoglobulin (Ig)-like cell adhesion molecules.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $380.00

Price(USD) : $2790.00

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